Additional file 1. Primers used for bisulfite-based PCR with subsequent SNuPE and local deep bisulfite sequencing (MiSeq). For MiSeq universal TruSeq adaptor sequences were attached to the specific se
We performed UPLC-MS/MS to investigate global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines (97L and LM6 cells). We found that 5mC and 5hmC were frequently dec
Long-read sequencing provides valuable information on difficult-to-map genomic regions, which can complement short-read sequencing to improve genome assembly, yet limited methods are available to accu