A deep insight about the role of hydrogen cynamide in grape bud dormancy release by comparative transcriptomic and proteomic analysis

Hydrogen cyanamide (HC) is an agrochemical compound frequently used to break bud dormancy in grapevine grown under mild winter conditions all over the world. The present study was carried out to get a better understanding of the molecular mechanism associated with HC to release bud dormancy in grapevine using RNA-seq based transcriptomic and tandem mass tag (TMT) based proteomic analysis. Two samples (Control and Hydrogen cynamide treated at 50% bud break) of grapevine buds were selected, and total RNA was extracted from these samples for further library construction.