A comprehensive two-hybrid analysis to explore the Legionella pneumophila effector-effector interactome

Illumina data in support of a Legionella pneumophila effector-wide screen for physical interactions using Barcode Fusion Genetic-Yeast Two-Hybrid (BFG-Y2H). Using this approach, we screened for protein-proteininteractions amongst all known L. pneumophila effectors and several (28) components of the Dot/Icmtype IV secretion system (>167,000 protein combinations). Of the 52 protein interactionsidentified by this approach, 44 are novel protein interactions, including ten novel effector-effector interactions (doubling the number of known effector-effector interactions).