10x single-cell RNAseq profiling of influenza Virus infected Human Lung Tissue Explants (HLTE)

This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging. Overall design: Human Lung tissue explant from COPD patients were cultrured in in vitro . These tissue pieces were infected with influenz A virus for 24 hours . After infection, tissue was digested into single cell suspension.