The pancreatic cancer stem cell marker CD44v6 affects transcription, translation and signaling: consequences on exosome composition and delivery

In cells recovery in wt, claudin7knockdown and palmitoylation site mutated claudin7 rescue was compared to elaborate the impact of claudin7 and palmitoylation site mutated claudin7 on miRNA recovery 2. In exosomes recovery in wt, claudin7knockdown and palmitoylation site mutated claudin7 rescue was compared to elaborate the impact of claudin7 and palmitoylation site mutated claudin7 in cells on the transport into exosomes during exosome biogenesis and the step of intraluminal vesicle loading. The comparison of the miRNA and proteins in cells allowed to judge on the impact of claudin7- and palmitoylation site mutated claudin7-related miRNA on protein recovery. The comparison of the miRNA and proteins in exosomes allowed to judge on the impact of claudin7- and palmitoylation site mutated claudin7-recruited miRNA on protein silencing in exosomes. The comparison between the impact of claudin7 and palmitoylation site mutated claudin7 on miRNA and proteins in cells versus exosomes allowed to judge on linked versus independent recruitment of miRNA and proteins and the impact of claudin7 on the recruitment of the mRNA processing machinery into exosomes. miRNA was analyzed in cells and exosomes of the rat pancreatic adenocarcinoma ASML wt, ASML-claudin7knockdown and ASML-claudin7kd cells rescued with claudin7 with a mutated palmitoylation site. miRNA analyses were compared with proteome analysis of the cell listed above.