16S rDNA-Seq, microbial diversity in Chinese paddy soil

The effects of green manures on the microbial community in six Chinese paddy were studied. The experiments located at agricultural experimental stations in four provinces in southern China: Hunan (29.37° N, 112.39° E), Jiangxi (28.19° N, 115.07° E), Anhui (30.66° N, 117.48° E), Henan (32.12° N, 114.08° E), Hubei (29.23° N, 113.08° E) and Fujian (26.08° N, 119.03° E). The joint experiment in the six site include for treatments: no fertilizer application as control (NF), green manure (GM) using milk vetch (Astragalus sinicus L.), chemical fertilizer only (CF), and a combination of chemical fertilizer and green manure (GMCF). All the soils at the six sites were sampled after the harvest of late rice in November and December in 2014. Three DNA extractions per sample were conducted using a FastDNA Spin Kit for Soil (MP Bio, Santa Ana, CA, USA) following the manufacturer’s procedures. The primer of 16S rDNA sequence is 338F (ACTCCTACGGGAGGCAGCA) and 806R (GGACTACHVGGGTWTCTAAT). The samples were run on Illumina MiSeq following the manufacturer’s instructions.