Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

The current study is aimed on determine the functional impact of microRNA-378a-3p and microRNA-146b on embryo development. For such purpose, we supplemented miR-378/miR-146b mimics or inhibitors to the culture medium containing presumed zygotes at 1 dpi and cultured them until day 8, thus allowing miR-378/miR-146b mimics or inhibitors to influence further embryo development and quality. Further on, to gain more in-depth molecular insights, we performed transcriptome profiling of blastocysts cultured in the presence of miR-378/miR-146b mimics or inhibitors. Overall design: Examining the effects of supplementing microRNA-378a-3p/146b mimics and inhibitors to embryos on the transcriptomic level