Overview of all sequenced libraries.

Duplex sequencing libraries for brain and muscle of 36 mice, liver of seven mice, and heart of one mouse, as well as 92 single oocytes and 24 oocyte pools. A total of five HiSeq 2500 runs were performed, labeled mm_DS01-mm_DS05. To increase the mtDNA sequencing depth, a second library was occasionally prepared for the same sample and sequenced in another run. For most single oocytes, enzymatic fragmentation, which is part of the NEB Ultra II FS DNA Library Preparation Kit, was used for DNA fragmentation (indicated with FS in column “fragmentation”); for somatic tissues, some single oocytes, and oocyte pools, shearing on a Covaris M220 machine was performed (indicated with CV in column “fragmentation”). Numbers of putative tissue-specific mutations are shown after filtering (as described in S1 Note). mtDNA, mitochondrial DNA. (XLSX)