Chromomeres represent universal units of chromatin packaging in lampbrush chromosomes. To extend our knowledge on chromomere organization, we applied microdissection to chicken lampbrush chromosomes. In particular, 31 and 5 individual chromomeres were dissected one by one along the macrochromosome 4 and one microchromosome, respectively. The data on genomic context of individual chromomeres was obtained by high-throughput sequencing of the corresponding chromomere DNA.. Lampbrush chromomeres microdissection and high-throughput sequencing

To extend our knowledge on lampbrush chromomere organization and genomic context, we performed microdissection of all prominent chromomeres from lampbrush macrochromosome 4 and one of the microchromosomes in a chicken lampbrush chromosome set. The data on genomic features of individual chromomeres were obtained by high-throughput sequencing. The DNA-library preparation was performed according to the manufacturer’s recommendations with some modifications (Ion Torrent, Life Technologies). In particular, primary DOP-PCR products of the dissected material were re-amplified and barcoded using a panel of Ion-Torrent primers. The sequencing data was processed and analyzed using the web-based bioinformatic platform Galaxy (https://usegalaxy.org/, Giardine et al., 2005) as earlier described (Zlotina et al., 2016). Analysis of genomic features showed that the majority of chromomere-loop complexes combine gene-dense and gene-poor regions, while massive loopless DAPI-positive chromomeres lack genes and are remarkably enriched with different repetitive elements.