Wnt-pathway genetic lesions in MSI colon tumors and cell lines.

NKD1, AXIN2, TCF7L2, CTNNB1, and APC gene mutation status in 40 MSI-CRC tumors (top) and 11 cell lines (bottom). Key: −, no lesion; ins, nucleotide insertion; del, nucleotide deletion; /−, allelic deletion. For NKD1 and AXIN2, mutation in indicated nucleotide is designated, while for TCF7L2 the status of the poly(A) tract (A8–wild-type; A9–mutant) is designated. CTNNB1 exon-3 was screened for activating mutations (M). For APC, tumors positive for protein-truncation (T) are indicated [45]. Presence (+) or absence (−) of APC and CTNNB1 lesions in each cell line is as described [48].