Saw Kill river (NY, USA) metagenomics and environmental variables

Microbial community structure and diversity in waterways are altered by
wastewater treatment plant (WWTP) discharge which can introduce
human-associated microbes, antimicrobial resistance (AMR), and
significantly change environmental variables. To better understand these
interactions, we investigated the impact of the Bard College WWTP on
microbial communities collected from the surface water and sediment from
four sites, two sites above and two sites below the Bard College outflow,
as well as the outflow itself, this was performed over a period of five
months. We measured physico-chemical parameters such as temperature,
turbidity, conductivity, dissolved oxygen, and salinity as well as the
bioindicators Escherichia coli, total coliforms, and Enterococcus sp.
concentration, endotoxins, the intI1 gene marker, and the total bacterial
abundance through 16S rRNA gene.