Putative photosensitivity in internal light organs (organs of Pesta) of deep-sea sergestid shrimps

Many marine species can regulate the intensity of bioluminescence from their ventral photophores in order to counterilluminate, a camouflage technique whereby animals closely match the intensity of the downwelling illumination blocked by their bodies, thereby hiding their silhouettes. Recent studies on autogenic cuticular photophores in deep-sea shrimps indicate that the photophores themselves are light-sensitive. Here, our results further suggest photosensitivity in a second type of autogenic photophore, the internal organs of Pesta, found in deep-sea sergestid shrimps. Experiments were conducted ship-board on live specimens, exposing the animals to bright light, which resulted in ultrastructural changes that matched those seen in crustacean eyes during the photoreceptor membrane turnover, a process that is crucial for the proper functioning of photosensitive components. In addition, RNA-seq studies demonstrated the expression of visual opsins and phototransduction genes in organs of P..., Biological replicates of Parasergestes armatus (n=4) were immediately preserved in RNAlater and stored at -80 °C upon live collection. Organs of Pesta were carefully dissected in chilled RNAlater to prevent degradation of nucleic acids and homogenized in TRIzol reagent (ThermoFisher Scientific). Total RNA was extracted following recommendations in DeLeo et al. (2018) and mRNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Pippin Prep (Sage Science) was used for size selection on barcoded libraries and all samples (n=4) were sequenced on an Illumina HiSeq4000.
FastQC was used to assess the quality of raw sequencing data and adaptor sequences were trimmed with Trimmomatic v0.36 (parameters: adapter.clip 2:30:10:1:true, crop 135, headcrop 15, trim.leading 3, trim.trailing 3, sliding.window 4:20, min.read.length 36). Rcorrector and BBnorm were used to error-correct reads and normalize read coverage. Tissue-specific transcriptomes were assem..., Alignment viewer (i.e., Aliview or Genewiz) and/or a Text editor, # Title of Dataset: Putative photosensitivity in internal light organs (organs of Pesta) of deep-sea sergestid shrimps

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This dataset contains a tissue-specific transcriptome (organs of Pesta) assembled de novo from replicate (n=4) RNAseq data for the deep-sea sergestid shrimp *Parasergestes armatus*. The organs of Pesta are a type of type of autogenic photophore, with our results indicating putative photosensitivity in addition to light emission.

This dataset also contains the putative visual opsin genes curated from the assembly using phylogenetically informed annotation tools, and associated alignment files used in subsequent analyses. These data support additional lines of evidence presented in the paper that the organs of Pesta are light sensitive.

## Description of the Data and file structure

trinity_Parmatus_organsPesta.fasta - Tissue-specific de novo transcriptome assembly file generated from organs of Pesta, autogenic photophores, from the deep-sea sergestid shrimp Paras...