Mcherry Fluorescence Correlation With Western Blot Band Intensity
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<strong>Overview</strong> The results present in this dataset were obtained from recombinant <em>Chlamydomonas reinhardtii</em> expressing the fluorescent protein mCherry in different constructs formats, aiming to recombinant protein secretion. Image present in this dataset were obtained by following the steps described in the protocol dx.doi.org/10.17504/protocols.io.kfpctmn. Fluorescence measurements were obtained by growing cc1690 in 50 mL TAP media on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m<sup>2</sup>s). Then, 100 μL of each strain was transferred to a clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland). Fluorescence was measured at excitation 575/9 nm and emission 608/20 nm. Supernatant samples were obtained by spinning 1 mL sample at 15000 × <em>g</em> for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement. <strong>Files Info</strong> 021115_20171219 Correlation_Fluore_vs_Densito_mCherry_50mL_7_days.xlsx -> data points for fluorescence measurements and pixel intensity obtained with FiJI (Fiji Is Just ImageJ). 022615 WB SP mCHerry Lysate 3.JPG -> Raw western blot image for lysate samples 022615 WB SP mCHerry Supernatant 3 .JPG -> Raw western blot image for supernatant samples 20171219 Fluor_Densito_Correlation_Lys.png -> Correlation plot of fluorescence and pixel intensity for lysate samples WB_Lysa_7days_for_Densitom.png -> Prepared image for densitometry analysis of lysate samples 20171219 WB_Sup_7days_for_Densitom.png -> Correlation plot of fluorescence and pixel intensity for supernatant samples WB_Sup_7days_for_Densitom.png -> Prepared image for densitometry analysis of supernatant samples <strong>Consider citing our work. </strong> Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433<br>
