Oligonucleotide primers used in this study.
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a Sourced from Choi and Schweizer, 2005.
bgene-UpF: Regular 18–20bp forward primer upstream of the target gene
cgene-UpR-Gm: 18–20bp reverse primer upstream of the target gene incorporating a 20–25bp overlap homologous to the 5’ end of the gentamicin resistance cassette in pPS856
dgene-DnF-Gm: 18–20bp forward primer downstream of the target gene incorporating a 20–25bp overlap homologous to the 3’ end of the gentamicin resistance cassette in pPS856
egene-DnR: Regular 18–20bp forward primer downstream of the target gene.
Oligonucleotide primers used in this study.
创建时间:
2015-08-07



