Transcriptomic choreography in response to light quality variation reveals metabolic acclimation in marine Nannochloropsis oceanica.

We report the transcriptomic dynamics and metabolic adaptation of Nannochloropsis oceanica to blue (410nm) and red (680nm) light by high-throghout Next-generation sequencing (NGS). Preferential excitation of different light spectra induces massive reprogramming of the Nannochloropsis transcriptome. Overall design: Nannochloropsis oceanica IMET1 was inoculated into modified f/2 liquid medium containing 30 g/L sea salt, 10 mM Tris-HCl (pH 7.6), 427.5 mg/L NaNO3, 30 mg/L NaH2PO4·H2O, 5 mg/L trace metal mixture (4.36 g/L Na2EDTA·2H2O, 3.15 g/L FeCl3·6H2O, 10 mg/L CoCl2·6H2O, 22 mg/L ZnSO4·7H2O, 180 mg/L MnCl2·4H2O, 9.8 mg/L CuSO4·5H2O, 6.3 mg/L Na2MoO4·2H2O), and 2.5 mg/L vitamin stock solution (1 mg/L vitamin B12, 1 mg/L biotin, 200 mg/L thiamine). The cells were cultivated in liquid cultures using column bioreactor under continuous light (approximately 80 ± 5 µmol photons m-2 s-1) at 25°C and aerated by bubbling with air-level CO2 (0.04%) at 0.6 vvm (volume gas per volume medium per minute) under white, red and blue light..After the switch from white light to red and blue light, cell aliquots were taken at 0 (white light), 12 (red and blue light), and 24h (red and blue light) from each column by syringe for transcriptomic profiling.