Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions
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https://www.ncbi.nlm.nih.gov/sra/SRP285110
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资源简介：
We develop CoTECH, a combinatorial barcoding method allowing for high-throughput parallel single-cell detection of chromatin occupancy and transcriptome. Overall design: Protein A-Tn5 was enriched through specific antibodies to genomic regions and Tn5 generated indexed chromatin. Following tagmentation, mRNA inside cells were captured by oligo dT barcode primers and reversed-transcribed. Combinatorial indexing strategy was implemented on both ChIPed DNA parts and RNA parts.
创建时间：
2021-06-05
