Rates of spontaneous translocation assessed during the serial growth of wild type and est2Δ/est2Δ mutant diploidsa.

aRates were determined by the method of the median (Lea and Coulson 1949) from at least 12 independent cultures. Fold differences from the rate obtained with the wild type strain with the same substrate locations and at equivalent time-points are indicated in parentheses. The 95% confidence intervals were determined and are indicated in brackets. Pre-senescence corresponds to approximately 25 generations, senescence corresponds to approximately 60 generations, and post-senescence corresponds to approximately 100–125 generations.bTranslocation substrates were as described in the Materials and Methods.