LPAAT-β siRNA treatment results in increased nuclear eccentricity.

MiaPaCa2 cells were treated (A) for 24 hr with 500 nM Torin-1 (* p < 0.0001), 10 nM rapamycin, or DMSO vehicle and (B) for 72 hr with 25 nM LP-4 (* p = 0.05) or non-targeting (nt) siRNA. Nuclei were stained with antibody to Lamin A to highlight the nuclear membrane as well as counterstained with DAPI. Analysis of the images using Definiens Tissue Studio software generated the length and width of individual nuclei, which were then analyzed for enhanced nuclear eccentricity as described in Materials and Methods. Graphs show the frequency distribution of nuclear eccentricity and nuclear l/w normalized to cell number. Statistical significance was determined using Student’s t-test. Results are representative of four independent experiments.