Live-cell STED dataset of mitochondria containing ground truth and corresponding low intensity noisy images

The dataset was acquired as part of the manuscript "Denoising diffusion models for high-resolution microscopy image restoration". The dataset contains ground truth and low intensity STED images of mitochondria acquired in live U2-OS cells stably expressing TOM20 coupled to the dead mutant of HaloTag7 which was made fluorescent by using the exchangeable ligand Hy4 bound to the fluorophore SiR.