Transgenerational herbivory stress response on DNA methylation in Thlaspi arvense

We investigated the maternal effects associated with herbivory across generations of plants to evaluate the response in methylation levels of damaged plants than undamaged plants. First, I evaluated DNA methylation variation of pennycress (Thlaspi ravens) damaged by herbivores (Pieris rapae) treated with a chemical elicitor of induced resistance (jasmonic acid) during the vegetative growth stage, induced resistance of the plants' progeny after getting exposed by Pieris rapae, Spodoptera exigua compared to controls. The effects of the herbivory treatments were assessed by quantifying genome-wide global DNA cytosine methylation and differentially methylated cytosines using epiGBS