Cerebellar Purkinje cell whole cell voltage clamp mIPSC recordings in Dag1 conditional mutant mice

Name: Cerebellar Purkinje cell whole cell voltage clamp mIPSC recordings in Dag1 conditional mutant mice
Creator: figshare
Published: 2025-06-05 23:05:52
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DataCite Commons2025-06-05 更新2025-09-08 收录

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https://figshare.com/articles/dataset/Cerebellar_Purkinje_cell_whole_cell_voltage_clamp_mIPSC_recordings_in_Dag1_conditional_mutant_mice/29083331/1

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Here we studied the role of Dystroglycan in the function of inhibitory cerebellar synapses from MLIs onto Purkinje cells. In all cases, we recorded mIPSCs from Purkinje cells held at -70mV in whole cell voltage clamp. The bath contained NBQX and TTX. Recordings were performed using Igor Pro 8 (Wavemetrics) and files are in the proprietary .pxp format. Genotype information for each recording can be found in the phys_metadata.csv file in this dataset. Igor code for mIPSC analysis can be found at https://github.com/jnjahncke/mini_analysis (for publication, a cutoff of -12000 was used).We performed experiments under 5 different genetic conditions:Calb1Cre;Dag1cKO - Dag1 deletion starting ~E14 (recordings performed at P30)Calb1Cre;Pomt2cKO - Loss of Dag1 glycosylation starting ~E14 (recordings performed at P30)Calb1Cre;Dag1cΔICD - Loss of Dag1 intracellular domain starting ~E14 (recordings performed at P30)Pcp2Cre;Dag1cKO - Dag1 deletion starting ~P14 (recordings performed at P30)Pcp2Cre;Dag1cKO - Dag1 deletion starting ~P14 (recordings performed at P60)

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figshare

创建时间：

2025-05-16