five

Data from: The palaeogenetics of cat dispersal in the ancient world

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Mendeley Data2024-06-25 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.g4p30
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Cat mtDNA fragmentsNine ND5 gene fragments were amplified via multiplex PCR and either sequenced by NGS in bulk following the “aMPlex Torrent” workflow, or reamplified individually by nested PCR and sequenced by pyrosequencing (see Ottoni et al, 2017, Nature Ecol Evol in press). The nine sequences obtained by “aMPlex Torrent” for each specimen were concatenated using a 10 N intervening spacer. For the specimen sequences that were obtained only by pyrosequencing, the N spacer was extended to allow alignment of all sequences. The coordinates of the fragments, excluding primers, numbered according to the Felis silvestris mtDNA reference sequence (NC001700) are: Frag_7: 12918-12961; Frag_2: 13052-13102; Frag_8: 13263-13333; Frag_3: 13513-13569; Frag_4: 13746-13807; Frag_1: 13945-13980; Frag_5: 14004-14012; Frag_9: 14170-14228; Frag_6: 15071-15131.cats_TOTAL_to_deposit_mtDNA_final.fastaCat Taqpep gene fragmentsThree fragments allowing genotyping of the T139, D228 and W841 codons of the Taqpep genes controlling the Macquerel or Blotched Tabby coat color pattern were concatenated for each specimen using a 10 N intervening spacer. The coordinates of the fragments, excluding primers, numbered according to the Felis silvestris Taqpep gene (LOC101101437) from the genomic sequence NC018723 are: T139: 21887-21926; D228: 22154-22180; W841: 80673-80731.cats_TOTAL_to_deposit_NUCLEAR_final.fasta
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2023-06-28
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