1Each peptide consists of a variant trimer (as listed in the column) between consistent trimers of glycine with a flourophore and quencher (MCA-GGG XXX GGG-DNP). 2min−1 µg CtHtrA−1 mean and (standard
C141S is a substitution derivative of AprV2 in which one disulphide bond has been removed. AprB2 is equivalent to AprV2.Y92R. Experiments were conducted at 25°C using 1 µM purified protease.
