Transcriptome-wide mapping of MARF1-bound mRNAs
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https://www.ncbi.nlm.nih.gov/sra/SRP260015
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资源简介：
To comprehensively identify MARF1-associated mRNAs, we performed iCLIP using engineered HEK293 cells stably expressing a doxycycline (Dox)-inducible FLAG-tagged MARF1 that lacks RNAse activity. Our iCLIP analysis of MARF1 in HEK293 cells identified 108 mRNAs bound by MARF1 and demonstrates that MARF1 binds to the majority of these mRNAs via their 3'UTRs. Overall design: UV cross-linking with immunoprecipitation using FLAG antibody, followed generating a library of MARF1-protected RNA fragments.
创建时间：
2020-06-20
