OD.xlsx

The MIC values were determined using a 96-well Microtiter plate assay. Antibiotics were prepared after sugested concentrations of J.M. Andrews (2001). The plate layout used for the MIC tests were all the same. Bacteriocins in row A-D and antibiotics in row E-H, The test solutions were undiluted in column 1 and two-fold dilutions were made from column 2-10. Column 11 and 12 were controls, a growth control with inoculated Müller-Hinton broth (MHB) and a sterility control with MHB. The pathogens were taken from glycerol stocks and were incubated in MHB overnight, except S. pneumoniae which were incubated for 6h. After incubation OD450 were measured and the cultures were diluted in an appropriate volume of MHB to a OD450 = 0, 01. Each well in column 1-11 were inoculated with the same pathogen and covered with film. Each plate setup was done in triplets and incubated at 37oC for 16h. After incubation OD450 were measured for further analysis.