The microscopic agglutination test (MAT) used for the serological diagnosis of leptospirosis, as a robust and inexpensive method, is still the reality in many laboratories worldwide. Both the performa
NA = not applicable. *Acute-phase patients with negative MAT result were diagnosed by seroconversion; convalescent patients with negative MAT result were diagnosed based on the acute MAT titer.
Strains, serogroups and serovars of Leptospira spp. used as live antigens in the Microscopic Agglutination Test (MAT) for the populations in the Netherlands (N = 14), and Colombia (N = 156).