Functional significance of the HIV-1 Tat signature amino acid residues

We sequenced the mRNA from a stably transduced Human T-cell line expressing the HIV-1 Tat protein. The inducible Tat expression has been used to understand and compare the cellular molecules and pathways being modulated by the wild type subtype-C Tat versus its single signature amino acid residue variant. Additionaly, the HIV-1 subtype B and C Tat comparitive analysis has also been included in the study to evaluate the gene expression profile as a reponse to subtype specific Tat proteins. Evaluation of the genes and pathways differentially modulated by the epression of the variant HIV-1 Tat proteins or suntype specific Tat proteins in a Human T-cell line using RNASeq analysis.