Kinetic parameters for wild-type enzyme and mutants using sucrose as substrate.

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Kinetic parameters of the purified wild-type and mutant SAPB enzymes with selected synthetic peptide substrates.

Assays were performed using the purified proteases in 100 mM buffer containing 2 mM Ca2+, and 0.2 mM to 50 mM synthetic peptide substrates (YLV and FAAF) at suitable pH. The samples were incubated for

NIAID Data Ecosystem40

Δ T m and kinetic parameters of wild-type BglA and mutant enzymes.

aTm of wild-type protein was detected to be 38.5°C. bSuggested mutation. n.d.: Not determined. ΔTm and kinetic parameters of wild-type BglA and mutant enzymes.

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