James R. LaFountain, Rudolf Oldenbourg (2011) CIL:10723, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Movie of anaphase B (spindle elongation) of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 70 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 4-frame algorithm (Shribak and Oldenbourg, 2003). The time series of images on which the movie is based is included in this grouped set.

本数据集记录了蜻蜓科昆虫Nephrotoma suturalis的初级精母细胞减数分裂I期的中期B期（纺锤体延长阶段）的动态变化过程。采用尼康Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，马萨诸塞州Woburn），搭载60x/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜以及2.0倍变焦镜头进行时间序列偏振显微摄影。图像采集频率为每30秒一次，连续采集70分钟，使用QImaging Retigo EXi CCD相机完成。原始图像经过4帧算法（Shribak and Oldenbourg，2003年）处理。本组集中包含了制作视频所依据的图像时间序列。