Effect of PIP4Ks on gene expression and alternative splicing levels in U2OS cells

Here we report the function of PIP4K isoforms in differential gene expression in physiological and UV conditions. Our data indicate that PIP4K2A knockdown adversely affects UV-induced gene expression and alternative splicing which is rescued by simultanous knockdown of PIP4K2B. Overall design: RNA-seq of U2OS cells containing wildtype ctrl; PIP4K2A knockdown ctrl; PIP4K2B knockdown ctrl; PIP4K2A/2B knockdown ctrl; wildtype UV; PIP4K2A knockdown UV; PIP4K2B knockdown UV; PIP4K2A/2B knockdown UV