Data set for Comprehensive Investigation of Matrix Effect Evaluation Approaches for Reliable Quantification in Bioanalysis using UHPLC-MS/MS

The main objective of this study was to systematically evaluate two quantitative approaches for matrix effect evaluation, i.e., the post-extraction addition and slope-based approach, using serum as a complex matrix and a non-selective protein precipitation procedure. Based on the observed limitations of slope-based evaluation, a novel intercept-based equation for the calculation of translational matrix effects was introduced. By combining translational matrix effects derived from calibration curve intercepts with rotational matrix effects derived from calibration curve slopes, the new approach provides a more reliable and comprehensive framework for ME evaluation. The accuracy and applicability of the new approach were subsequently demonstrated using a validation set of compounds analyzed in three different matrices on two various UHPLC–MS/MS platforms.