five

MSC-NK co-culture, D1, MSC3

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http://flowrepository.org/id/FR-FCM-ZY9V
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NK cells from an healthy donor (D1) were co-cultured for 4 days with human lung tumor-derived MSC and non-tumor MSC (patient 3), and activated toward K562 for 4 hours. NK degranulation (CD107a) and IFNgamma production were assessed by flow cytometry. Gating strategy for NK cells: CD45+CD3-CD56+CD16+/-.
创建时间:
2017-08-01
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