Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function. Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function

Purpose:RNA-seq was uesd to identify how sodium butyrate regulated murine Bregs differentiation. Methods:CD19+ B cells isolated from C57BL/6 mice spleen were were polarized to Bregs differentiation with LPS (10μg/ml) in the absence or presence of sodium butyrate (0.5mM) for 48 hours, and treated with PIM at the last 5 hours following RNA preparation. Results:Using an optimized data analysis workflow, we identified 1462 differentially expressed genes (DEG), of which 720 genes were up-regulated and 742 genes down-regulated, respectively (fold change >2 and adjust p-value 2 and adjust p-value 2 and adjust p-value < 0.05). RT-qPCR wasused to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that MAPK signaling pathway might involve the function of butyrate on Bregs,which had been proved by western blotting. Overall design: Bregs were were generated with LPS (10μg/ml) in the absence or presence of sodium butyrate (0.5mM) for 48 hours, and treated with PIM at the last 5 hours.