Quantitative (Glyco-)Proteomics of Haloferax volcanii Biofilm Formation

A quantitative proteomics analysis comparing planktonic and sessile cells at different stages of biofilm development, using iTRAQ labeling, has been performed for Haloferax volcanii. Samples were taken from liquid, shaking cultures were compared to samples from static cultures, separated into sessile (biofilm) and planktonic cells. This dataset resulted in the identification of more than 50% of the H. volcanii proteome. Most importantly, expression as well as N-glycosylation of various surface filaments (pili, flagella and SLG) has been quantified, strongly supporting the hypothesis, that differential N-glycosylation plays important roles in the biofilm formation of H. volcanii.