Examining Pathogenesis in Genome Instability Associated Neurodegenerative Mouse Model [TotalStranded-RNA-seq]

To investigate the molecular basis for ataxia in the DNA repair deficient mice we utilized RNA-seq to evaluate the transcriptome of AtmNes-cre;Aptx-/- (and AtmNes-cre;Parp1-/-) cerebellar and cortical tissue. Using total RNA from symptomatic 10-month-old double-mutant mice and littermate controls, we performed the transcriptome analysis. top 1,000 expressed genes in each genotype Overall design: Total Stranded RNA sequencing of the cerebellum and cortex examining gene expression in ATM APTX null and associated control brains.