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Assay conditions for the specific activity determination with alternative substrates.

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All measurements were performed in 0.1 M sodium phosphate buffer, pH 6.5 at 30°C. The stock solutions for Isothiocyanates were prepared in acetonitrile (2% final concentration in the assay), and that for CDNB in ethanol (5% final concentration in the assay). Assay conditions for the specific activity determination with alternative substrates.
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2014-10-16
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