Pbr1 Allele Alignment
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https://figshare.com/articles/dataset/Pbr1_Allele_Alignment/7315655
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资源简介：
Alignment of Pbr1 allele sequences (genomic DNA of start codon to stop codon) from 12 barley genotypes, aligned by Clustal Omega. Allele sequences were obtained by Sanger sequencing PCR fragments. This alignment was used to generate the phylogenetic tree in Figure 5C in Carter and Helm, et al 2018 (in submission).
From methods section:DNA was isolated from ground frozen leaf tissue using the GeneJET Plant Genomic DNA Purification
Kit (Thermo Scientific™).
Primers were designed throughout the genes of interest and fragments
were amplified from genomic DNA using Q5 2X Master Mix (NEB), then Sanger
sequenced at the Cornell Biotechnology Resource Center.For the analysis of Pbr1
alleles, nucleotide sequences were selected from each sequenced allele that
spanned from the start codon to the stop codon of the Rasmusson allele,
including the intron. Sequences were aligned with Clustal Omega.
创建时间：
2018-11-09
