Single Cell Sequencing Analysis of Infiltrating Immune Cells in Orthotopically Implanted Liver Cancer in Mice Treated with Isotype or Anti-PD-1 Therapy

The anti-PD-1 mAb is a type of immunotherapy known to have a significant impact on tumor immune components. In this study, we utilized single-cell RNA sequencing (scRNAseq) to analyze infiltrating immune cells of liver cancer in mice treated with both anti-PD-1 and isotype control. Overall design: Following treatment with either IgG or anti-PD-1, C57/BL6 mice with orthotopic liver tumors underwent Fluorescence-activated cell sorting (FACS) sorting of tumor-infiltrating CD45+ immune cells, which were subsequently subjected to scRNAseq.