Identification of STAMBP-interacting proteins by IP-MS screening.

To identify proteins interacting with STAMBP, we performed co-immunoprecipitation (CoIP) assays using pancreatic cancer cells. Specifically, SW1990 cells stably overexpressing either the empty vector control or HA-tagged STAMBP (HA-STAMBP) were subjected to CoIP with an anti-HA antibody to enrich HA-associated proteins. Proteins specifically interacting with HA-STAMBP were then identified by comparative proteomic analysis between the two cell lines. Mass spectrometry-based proteomic services were provided by Shanghai Omicsspace Biotech.