Complement activation induces excessive T cell cytotoxicity in severe COVID-19

We performed single cell transcriptomics in 13 acute and convalescent mild versus severe COVID-19 subjects, in healthy controls and in sujects with flu-like-illness and HBV infection to assess COVID-19-specific T cell populations und function. Blood from 19 COVID-19 patients at acute and convalescent (for 13 patients) phases for mild-moderate (9 patients) and severe (10 patients), from 6 healthy controls as well as from 8 patients with flu-like disease and 4 patients with chronic HBV infection was collected and PBMC prepared. From part of the PBMC CD38+ CD4+ an CD8+ were purified and both, total PBMC and enriched CD38+ T cells were subjected to 5' GEX scRNA- and 5'-VDJ library preparation. For many samples, both all PBMCs and enriched CD38+ T-cells were sequenced. Samples were pooled with Antibody-based Hashtags and demultiplexed. based on both Hashtags and SNPs. >>> Submitter declares raw data not available due to patient privacy concerns <<<