Rhythms of lysis: viral regulation of prokaryotic turnover across diel cycles

This dataset accompanies the manuscript “Rhythms of lysis: viral regulation of prokaryotic turnover across diel cycles” and contains all non-SRA data used in the analyses. Samples were collected at two oligotrophic time-series stations (SEATS and SS1) in the South China Sea during August 2023.Environmental_metadata.xlsx provides station, date and time, depth, latitude and longitude, hydrographic variables (temperature, salinity, dissolved oxygen), fluorescence, photosynthetically active radiation (PAR), nutrient concentrations (NO₂⁻, NO₃⁻, PO₄³⁻, SiO₃²⁻), and abundances of heterotrophic bacteria, Synechococcus and Prochlorococcus.Additional worksheets summarize the acrophases (peak times) of diel periodicity in cell abundances for different microbial groups at SEATS and SS1.ASV_metadata.xlsx contains 16S rRNA amplicon sequence variant (ASV) tables and taxonomy; rows are ASVs and columns are samples for the rDNAcell, rRNAcell and rRNAext fractions.Dilution experiment data.xlsx reports cell abundances of heterotrophic bacteria and Synechococcus for each dilution treatment and time point (0, 6, 12, 18, 24 h) in the modified dilution experiments at SEATS and SS1.group.txt lists the sample group assignments (e.g. LOWDAY, LOWNIGHT, HIGHDAY, HIGHNIGHT) used in downstream analyses.seven lysis group of ASV.R and WGCNA-diel ASVs.R are R scripts that reproduce the seven lysis-group classification, CLI calculations, WGCNA and the associated figures.