Supplemental Table S3
收藏DataCite Commons2020-12-28 更新2024-08-18 收录
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https://figshare.com/articles/dataset/Supplemental_Table_S3/13355939
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Primers used to amplify wild-type genes corresponding to transposon mutants and clone them into pOS1-P<i>lgt</i> with splicing overlap extension (SOE)-PCR or HiFi/Gibson assembly. The annealing portion is bolded. Tms were calculated for NEB Q5 HF polymerase (https://tmcalculator.neb.com/#!/batch). <i>fmtC</i> primers were used to amplify <i>fmtC</i> from USA300 and NRS209.
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figshare
创建时间：
2020-12-10
