Locomotor activity rhythms of flies with spag RNAi knockdown.

111 of 16 flies died during the locomotor assay.216 of 16 flies died during the locomotor assay.The timGAL4 or UAS-dcr; timGAL4 lines were crossed to the indicated responder line (stock number from VDRC or Bloomington Stock Center indicated), entrained to LD 12hr:12hr, and male progeny hemizyous for UAS-dcr (where indicated) and heterozygous for timGAL4 and the responder were collected after 18 days. These were immediately placed into activity assays that were monitored for one week in DD (1 week assays) or aged for 1 week and then monitored for another week in DD (2 week assays). The periods were determined by chi-square periodogram analysis, and rhythmic flies produced a single strong peak above the line of statistical significance (pLocomotor activity rhythms of flies with spag RNAi knockdown.