Structural analysis of mutations associated with epidemic trajectory and microcephaly.

The percentage of mutations occurring in greater than 10% of ZIKV genomes (Asian Lineage) between 2010–2017, with at least 25 cases occurring in 2016, is shown in middle column “%(2006–17)”. Mutations that are predicted by protein structural models to occur in exposed regions of the protein are marked as a “1” in the “Exposed” column, and mutations predicted to occur in buried regions are marked as “0”. Cells with the 5 mutations that occurred in 2013 (as shown in Fig 1) are red, and cells representing the 2014 NS5 Thr114Val mutation are light purple. Cells with Cluster 1.1 mutation NS1 Met349Val are pink, and Cluster 1.2 mutations and Cluster 1.3 mutations are in blue and green cells, respectively. Mutations that are found in the whole genome sequences of all known microcephaly cases are in bold italicized text in the “Epi Mut” column and bold black text in this column marks mutations observed in at least 1 microcephaly case. Black underlined text marks mutations that are not observed in genome sequences from any microcephaly cases.