LIC cloning of inverted repeats into three different vectors.
收藏Figshare2015-12-02 更新2026-04-29 收录
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Successful cloning is based on the availability of the desired target sequences, so failure to amplify the target sequence is a PCR failure and is not considered a cloning failure. The cloning success rate refers to the number of the desired LIC constructs attained following one round of cloning; this includes incorporation of the uracil containing primers, addition of the loop, and generation of the ss tails and annealing to the vector in the desired orientation.
创建时间：
2015-12-02
