Dissociation of free ubiquitinated and phosphorylated ARM from SLMB and the 'destruction complex'

The bound and phosphorylated Armadillo (ARM) is ubiquitated by the ubiquitin ligase assembled complex, whether this occurs when ARM is bound to the 'destruction complex' or after it has dissociated from it is unclear. The interaction between Slimb (SLMB) and ARM has weakened the latter's binding to the scaffolding molecules in the 'destruction complex'. ARM and the bound ubiquitin ligase assembled complex dissociate from Axin (AXN), APC (APC/APC2) and also from each other to leave free ubiquitinated and phosphorylated ARM which is now marked for degradation by the 26S proteasome.

臂形蛋白（ARM）在与其‘降解复合体’结合或从其解离后，均会被泛素连接酶组装复合体泛素化，然而这一过程是在ARM与‘降解复合体’结合时发生，还是在其解离之后发生尚不明确。SLIMB（SLMB）与ARM的相互作用削弱了ARM与‘降解复合体’中支架分子的结合。ARM及其结合的泛素连接酶组装复合体从Axin（AXN）、APC（APC/APC2）以及彼此中解离，留下自由泛素化和磷酸化的ARM，此时ARM被标记为26S蛋白酶体降解。