Quantifying the effects of 48 alleles of the TDH3 promoter on the mean activity and the variability of activity (noise) of the promoter. These promoter variants were cloned upstream of a YFP reporter
Enhancers function with a basal promoter to control the transcription of target genes. Enhancer regulatory activity is often studied using reporter-based transgene assays. However, unmatched results h
Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostl
Laccase content was determined under the influence of different concentrations of Triton X-100, and the key enzyme genes were cloned, and finally the expression of laccase was improve by strong promo