Reduced representation bisulfite sequencing (RRBS) profiles of human induced pluripotent stem cells exposed to varied chemicals

We studied the effects of chemical exposure on global DNA methylation by using mCherry-MBD-nls human iPS cells. By using 135 chemical library, we could devide the effects of global DNA methylation into 3 categories; reduction (hypomethylation), increase (hypermethylation) and little/no effect. We further performed both genome-wide DNA methylation and stemness-related gene expression (100 genes) analyses, and results indicated that hypermethylation agents increased DNA methylation and downregulated the expression of genes involved in cell cycle or development. Exposure to DMSO, etoposide, propyl gallate, theophylline, or 5-aza-dc for 48 hrs.