Gene expression profile at single cell level of HS and normal skin

We used single cell RNA sequencing (scRNA-seq) to analyze infiltration and diversity of various immune and other types of cell populations. The Institutional Review Board of the University of Alabama at Birmingham approved the protocol (IRB-300005214) for obtaining surgically discarded skin tissues from heathy and HS (Hurley II/III stage) subjects. Surgical excisions from 6 patients with HS (axillary and groin lesions) and 2 healthy controls (discarded tissue from breast or abdominoplasty reduction surgery) were collected.