Mapping and quantification quality Based on the GRCH38 reference genome and gene annotation

In all plots, colors represent different sequencing batches. (A) Proportion of clean reads successfully mapped to the Grch38 human reference genome. (B) Percentage of mapped reads to multiple regions (two or more) on the reference genome. (C) Proportion of reads quantified as specific transcripts based on Ensembl gene annotation. (D) Number of reads successfully quantified across entire samples.